dbp antibodies Search Results


93
Santa Cruz Biotechnology protein vdbp
Protein Vdbp, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Proteintech dbp
Dbp, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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93
Proteintech antiddx1
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Proteintech anti gnpat
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Proteintech protein vdbp polyclonal antibody
Dynamic changes in differential protein expression in cardiac tissues among the control, 1st and 14th day groups.
Protein Vdbp Polyclonal Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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85
Aviva Systems antibodies against mouse dbp
Dynamic changes in differential protein expression in cardiac tissues among the control, 1st and 14th day groups.
Antibodies Against Mouse Dbp, supplied by Aviva Systems, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Aviva Systems dbp
Dynamic changes in differential protein expression in cardiac tissues among the control, 1st and 14th day groups.
Dbp, supplied by Aviva Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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86
Aviva Systems rabbit anti dbp antibody
Attenuation of <t>DBP</t> transcriptional activity induced by chronic ER stress. (a) The Dbp <t>and</t> <t>Nfil3/E4bp4</t> mRNA expression levels in Wfs1 +/+ a/a, Wfs1 +/+ A y /a , Wfs1 −/− a/a and Wfs1 −/− A y /a islets at ZT12. Data are presented as relative expressions, and each point represents the mean + SEM (N = 3/group; ★★ p < 0.01). (b–c) MIN6 cells (b) and mouse islets (c) were incubated with thapsigargin at the indicated concentrations for 24 h and then subjected to real-time RT-PCR. Data are expressed relative to the values for cells incubated without thapsigargin and are the means + SEM of six experiments. ★ p < 0.05, ★★ p < 0.01. (d) Mouse islets were incubated with tunicamycin at the indicated concentrations for 24 h and then subjected to real-time RT-PCR. Data are expressed relative to the values for cells incubated without tunicamycin and are the means + SEM of six experiments. ★★ p < 0.01.
Rabbit Anti Dbp Antibody, supplied by Aviva Systems, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
Fapon Biotech monoclonal antibody to dbp
Attenuation of <t>DBP</t> transcriptional activity induced by chronic ER stress. (a) The Dbp <t>and</t> <t>Nfil3/E4bp4</t> mRNA expression levels in Wfs1 +/+ a/a, Wfs1 +/+ A y /a , Wfs1 −/− a/a and Wfs1 −/− A y /a islets at ZT12. Data are presented as relative expressions, and each point represents the mean + SEM (N = 3/group; ★★ p < 0.01). (b–c) MIN6 cells (b) and mouse islets (c) were incubated with thapsigargin at the indicated concentrations for 24 h and then subjected to real-time RT-PCR. Data are expressed relative to the values for cells incubated without thapsigargin and are the means + SEM of six experiments. ★ p < 0.05, ★★ p < 0.01. (d) Mouse islets were incubated with tunicamycin at the indicated concentrations for 24 h and then subjected to real-time RT-PCR. Data are expressed relative to the values for cells incubated without tunicamycin and are the means + SEM of six experiments. ★★ p < 0.01.
Monoclonal Antibody To Dbp, supplied by Fapon Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Pacific Immunology rabbit polyclonal antibodies for hadv-b7 and b14 e1a and dbp proteins
Attenuation of <t>DBP</t> transcriptional activity induced by chronic ER stress. (a) The Dbp <t>and</t> <t>Nfil3/E4bp4</t> mRNA expression levels in Wfs1 +/+ a/a, Wfs1 +/+ A y /a , Wfs1 −/− a/a and Wfs1 −/− A y /a islets at ZT12. Data are presented as relative expressions, and each point represents the mean + SEM (N = 3/group; ★★ p < 0.01). (b–c) MIN6 cells (b) and mouse islets (c) were incubated with thapsigargin at the indicated concentrations for 24 h and then subjected to real-time RT-PCR. Data are expressed relative to the values for cells incubated without thapsigargin and are the means + SEM of six experiments. ★ p < 0.05, ★★ p < 0.01. (d) Mouse islets were incubated with tunicamycin at the indicated concentrations for 24 h and then subjected to real-time RT-PCR. Data are expressed relative to the values for cells incubated without tunicamycin and are the means + SEM of six experiments. ★★ p < 0.01.
Rabbit Polyclonal Antibodies For Hadv B7 And B14 E1a And Dbp Proteins, supplied by Pacific Immunology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
MBL Life science anti-dbp antibody pm079
Attenuation of <t>DBP</t> transcriptional activity induced by chronic ER stress. (a) The Dbp <t>and</t> <t>Nfil3/E4bp4</t> mRNA expression levels in Wfs1 +/+ a/a, Wfs1 +/+ A y /a , Wfs1 −/− a/a and Wfs1 −/− A y /a islets at ZT12. Data are presented as relative expressions, and each point represents the mean + SEM (N = 3/group; ★★ p < 0.01). (b–c) MIN6 cells (b) and mouse islets (c) were incubated with thapsigargin at the indicated concentrations for 24 h and then subjected to real-time RT-PCR. Data are expressed relative to the values for cells incubated without thapsigargin and are the means + SEM of six experiments. ★ p < 0.05, ★★ p < 0.01. (d) Mouse islets were incubated with tunicamycin at the indicated concentrations for 24 h and then subjected to real-time RT-PCR. Data are expressed relative to the values for cells incubated without tunicamycin and are the means + SEM of six experiments. ★★ p < 0.01.
Anti Dbp Antibody Pm079, supplied by MBL Life science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
Gallus Immunotech chicken anti-human dbp antibody
Attenuation of <t>DBP</t> transcriptional activity induced by chronic ER stress. (a) The Dbp <t>and</t> <t>Nfil3/E4bp4</t> mRNA expression levels in Wfs1 +/+ a/a, Wfs1 +/+ A y /a , Wfs1 −/− a/a and Wfs1 −/− A y /a islets at ZT12. Data are presented as relative expressions, and each point represents the mean + SEM (N = 3/group; ★★ p < 0.01). (b–c) MIN6 cells (b) and mouse islets (c) were incubated with thapsigargin at the indicated concentrations for 24 h and then subjected to real-time RT-PCR. Data are expressed relative to the values for cells incubated without thapsigargin and are the means + SEM of six experiments. ★ p < 0.05, ★★ p < 0.01. (d) Mouse islets were incubated with tunicamycin at the indicated concentrations for 24 h and then subjected to real-time RT-PCR. Data are expressed relative to the values for cells incubated without tunicamycin and are the means + SEM of six experiments. ★★ p < 0.01.
Chicken Anti Human Dbp Antibody, supplied by Gallus Immunotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Dynamic changes in differential protein expression in cardiac tissues among the control, 1st and 14th day groups.

Journal: Molecular Medicine Reports

Article Title: Proteomics of acute heart failure in a rat post-myocardial infarction model

doi: 10.3892/mmr.2017.6820

Figure Lengend Snippet: Dynamic changes in differential protein expression in cardiac tissues among the control, 1st and 14th day groups.

Article Snippet: The membranes were probed overnight at 4°C with rabbit anti-myosin 7 polyclonal antibody (cat. no. 22280-1-AP; ProteinTech Group, Inc., Chicago, IL, USA), rabbit anti-Vitamin D binding protein (VDBP) polyclonal antibody (cat. no. 16922-1-AP; ProteinTech), rabbit anti-gelsolin polyclonal antibody (cat. no. PB0198; Wuhan Boster Biological Technology, Ltd., Wuhan, China) and rabbit anti-Voltage-dependent L-type calcium channel subunit α1D polyclonal antibody (cat. no. PB0286; Wuhan Boster Biological Technology, Ltd.).

Techniques: Expressing, Control, Binding Assay

Western blot analysis to validate the alterations in the selected proteins identified by proteomic analysis. (A) Cell lysates were extracted from the left ventricle of untreated (control), 1st day post-MI and 14th day post-MI rats and analyzed by western blotting prior to quantification of (B) Myosin-7, (C) Gelsolin, (D) VDBP and (E) Cav1.3 protein expression. GAPDH was used to normalize expression levels. All experiments were performed at least twice. Data are presented as the mean ± the standard deviation. **P<0.01. VDBP, vitamin D binding protein; Cav1.3, voltage-dependent L-type calcium channel subunit α1D; MI, myocardial infarction; control group, operation with no occlusion (untreated); 1st day group, tissues were taken from rats 1 day following surgical procedures; 14th day group, tissues were taken from rats 14 days following surgical procedures.

Journal: Molecular Medicine Reports

Article Title: Proteomics of acute heart failure in a rat post-myocardial infarction model

doi: 10.3892/mmr.2017.6820

Figure Lengend Snippet: Western blot analysis to validate the alterations in the selected proteins identified by proteomic analysis. (A) Cell lysates were extracted from the left ventricle of untreated (control), 1st day post-MI and 14th day post-MI rats and analyzed by western blotting prior to quantification of (B) Myosin-7, (C) Gelsolin, (D) VDBP and (E) Cav1.3 protein expression. GAPDH was used to normalize expression levels. All experiments were performed at least twice. Data are presented as the mean ± the standard deviation. **P<0.01. VDBP, vitamin D binding protein; Cav1.3, voltage-dependent L-type calcium channel subunit α1D; MI, myocardial infarction; control group, operation with no occlusion (untreated); 1st day group, tissues were taken from rats 1 day following surgical procedures; 14th day group, tissues were taken from rats 14 days following surgical procedures.

Article Snippet: The membranes were probed overnight at 4°C with rabbit anti-myosin 7 polyclonal antibody (cat. no. 22280-1-AP; ProteinTech Group, Inc., Chicago, IL, USA), rabbit anti-Vitamin D binding protein (VDBP) polyclonal antibody (cat. no. 16922-1-AP; ProteinTech), rabbit anti-gelsolin polyclonal antibody (cat. no. PB0198; Wuhan Boster Biological Technology, Ltd., Wuhan, China) and rabbit anti-Voltage-dependent L-type calcium channel subunit α1D polyclonal antibody (cat. no. PB0286; Wuhan Boster Biological Technology, Ltd.).

Techniques: Western Blot, Control, Expressing, Standard Deviation, Binding Assay

Immunohistochemistry validations of the alterations in the selected proteins identified by proteomic analysis. Myocardial tissues from untreated (control), 1st day post-MI and 14th day post-MI rats were embedded in conventional paraffin and the expression of Myosin-7, VDBP, Gelsolin and Cav1.3 were detected by immunohistochemistry staining using the appropriate antibodies (magnification, ×400). VDBP, vitamin D binding protein; Cav1.3, voltage-dependent L-type calcium channel subunit α1D; MI, myocardial infarction; control group, operation with no occlusion (untreated); 1st day group, tissues were taken from rats 1 day following surgical procedures; 14th day group, tissues were taken from rats 14 days following surgical procedures.

Journal: Molecular Medicine Reports

Article Title: Proteomics of acute heart failure in a rat post-myocardial infarction model

doi: 10.3892/mmr.2017.6820

Figure Lengend Snippet: Immunohistochemistry validations of the alterations in the selected proteins identified by proteomic analysis. Myocardial tissues from untreated (control), 1st day post-MI and 14th day post-MI rats were embedded in conventional paraffin and the expression of Myosin-7, VDBP, Gelsolin and Cav1.3 were detected by immunohistochemistry staining using the appropriate antibodies (magnification, ×400). VDBP, vitamin D binding protein; Cav1.3, voltage-dependent L-type calcium channel subunit α1D; MI, myocardial infarction; control group, operation with no occlusion (untreated); 1st day group, tissues were taken from rats 1 day following surgical procedures; 14th day group, tissues were taken from rats 14 days following surgical procedures.

Article Snippet: The membranes were probed overnight at 4°C with rabbit anti-myosin 7 polyclonal antibody (cat. no. 22280-1-AP; ProteinTech Group, Inc., Chicago, IL, USA), rabbit anti-Vitamin D binding protein (VDBP) polyclonal antibody (cat. no. 16922-1-AP; ProteinTech), rabbit anti-gelsolin polyclonal antibody (cat. no. PB0198; Wuhan Boster Biological Technology, Ltd., Wuhan, China) and rabbit anti-Voltage-dependent L-type calcium channel subunit α1D polyclonal antibody (cat. no. PB0286; Wuhan Boster Biological Technology, Ltd.).

Techniques: Immunohistochemistry, Control, Expressing, Staining, Binding Assay

Attenuation of DBP transcriptional activity induced by chronic ER stress. (a) The Dbp and Nfil3/E4bp4 mRNA expression levels in Wfs1 +/+ a/a, Wfs1 +/+ A y /a , Wfs1 −/− a/a and Wfs1 −/− A y /a islets at ZT12. Data are presented as relative expressions, and each point represents the mean + SEM (N = 3/group; ★★ p < 0.01). (b–c) MIN6 cells (b) and mouse islets (c) were incubated with thapsigargin at the indicated concentrations for 24 h and then subjected to real-time RT-PCR. Data are expressed relative to the values for cells incubated without thapsigargin and are the means + SEM of six experiments. ★ p < 0.05, ★★ p < 0.01. (d) Mouse islets were incubated with tunicamycin at the indicated concentrations for 24 h and then subjected to real-time RT-PCR. Data are expressed relative to the values for cells incubated without tunicamycin and are the means + SEM of six experiments. ★★ p < 0.01.

Journal: EBioMedicine

Article Title: Clock Gene Dysregulation Induced by Chronic ER Stress Disrupts β-cell Function

doi: 10.1016/j.ebiom.2017.03.040

Figure Lengend Snippet: Attenuation of DBP transcriptional activity induced by chronic ER stress. (a) The Dbp and Nfil3/E4bp4 mRNA expression levels in Wfs1 +/+ a/a, Wfs1 +/+ A y /a , Wfs1 −/− a/a and Wfs1 −/− A y /a islets at ZT12. Data are presented as relative expressions, and each point represents the mean + SEM (N = 3/group; ★★ p < 0.01). (b–c) MIN6 cells (b) and mouse islets (c) were incubated with thapsigargin at the indicated concentrations for 24 h and then subjected to real-time RT-PCR. Data are expressed relative to the values for cells incubated without thapsigargin and are the means + SEM of six experiments. ★ p < 0.05, ★★ p < 0.01. (d) Mouse islets were incubated with tunicamycin at the indicated concentrations for 24 h and then subjected to real-time RT-PCR. Data are expressed relative to the values for cells incubated without tunicamycin and are the means + SEM of six experiments. ★★ p < 0.01.

Article Snippet: Normal rabbit IgG (sc-2027; Santa Cruz), rabbit anti-RNA polymerase II (Millipore), rabbit anti-DBP antibody (AVIVA SYSTEM BIOLOGY), and anti-E4BP4 (sc-28,203; Santa Cruz) were used for immunoprecipitation.

Techniques: Activity Assay, Expressing, Incubation, Quantitative RT-PCR

DBP and E4BP4 directly regulate the transcription of genes linked to insulin secretion. (a) Real-time PCR analysis of clock-controlled genes, Slc2a2 , Ins1 , Ins2 and Rab37, in isolated islets during a 12 h light-dark cycle (N = 6 per time point). Data are means ± SEM. ★ p < 0.05, ★★ p < 0.01 (controls versus TG-C). (b) E4BP4 at the selected gene promoters in pancreatic islets isolated from TG-C mice. UCSC genome browser images of E4BP4 profiles at Ins1 , Ins2 , Slc2a2 and Rab37 in TG-C mice. Chromosome coordinates are indicated at the top. The plot in the middle shows the density of ChIP-seq reads with the peak score indicated on the Y-axis. The Ins1 , Ins2 , Slc2a2 and Rab37 genes are shown at the bottom. Red scale bars indicate 1 kb of genome. (c) Schematic models depicting the circadian control of insulin release via regulation of the basal ATP/ADP ratio and the gene expressions of Slc2a2 , Ins1 , Ins2 and Rab37 in islets.

Journal: EBioMedicine

Article Title: Clock Gene Dysregulation Induced by Chronic ER Stress Disrupts β-cell Function

doi: 10.1016/j.ebiom.2017.03.040

Figure Lengend Snippet: DBP and E4BP4 directly regulate the transcription of genes linked to insulin secretion. (a) Real-time PCR analysis of clock-controlled genes, Slc2a2 , Ins1 , Ins2 and Rab37, in isolated islets during a 12 h light-dark cycle (N = 6 per time point). Data are means ± SEM. ★ p < 0.05, ★★ p < 0.01 (controls versus TG-C). (b) E4BP4 at the selected gene promoters in pancreatic islets isolated from TG-C mice. UCSC genome browser images of E4BP4 profiles at Ins1 , Ins2 , Slc2a2 and Rab37 in TG-C mice. Chromosome coordinates are indicated at the top. The plot in the middle shows the density of ChIP-seq reads with the peak score indicated on the Y-axis. The Ins1 , Ins2 , Slc2a2 and Rab37 genes are shown at the bottom. Red scale bars indicate 1 kb of genome. (c) Schematic models depicting the circadian control of insulin release via regulation of the basal ATP/ADP ratio and the gene expressions of Slc2a2 , Ins1 , Ins2 and Rab37 in islets.

Article Snippet: Normal rabbit IgG (sc-2027; Santa Cruz), rabbit anti-RNA polymerase II (Millipore), rabbit anti-DBP antibody (AVIVA SYSTEM BIOLOGY), and anti-E4BP4 (sc-28,203; Santa Cruz) were used for immunoprecipitation.

Techniques: Real-time Polymerase Chain Reaction, Isolation, ChIP-sequencing